Fig. 4: MGP activates the calcium signaling pathway in MSCs. | Cell Death Discovery

Fig. 4: MGP activates the calcium signaling pathway in MSCs.

From: MGP regulates the adipogenic differentiation of mesenchymal stem cells in osteoporosis via the Ca2+/CaMKII/RIP140/FABP3 axis

Fig. 4

A The level of intracellular calcium ions in MSCs infected with sh-NC, sh-MGP, OE-NC, or OE-MGP lentiviruses was assessed through calcium fluorescence, and the quantitative data are shown. B The level of intracellular calcium ions in MSCs infected with sh-NC or shMGP was determined through flow cytometry using Fluo-4 AM. C Protein levels and quantification of MGP, CaMKII, P-CaMKII, and GAPDH expression after infection with OE-NC or OE-MGP lentiviruses and/or addition of the calcium ion chelator BAPTA-AM. Scale bar = 50 μm. All the data are presented as the means ± SDs; n = 6 per group. Student’s t test or ANOVA was used to determine significant differences. ns not statistically significant; *P < 0.05; **P < 0.01; and ***P < 0.001.

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