Fig. 5: RIP140 is involved in MGP-induced adipogenic differentiation through binding to the FABP3 promoter. | Cell Death Discovery

Fig. 5: RIP140 is involved in MGP-induced adipogenic differentiation through binding to the FABP3 promoter.

From: MGP regulates the adipogenic differentiation of mesenchymal stem cells in osteoporosis via the Ca2+/CaMKII/RIP140/FABP3 axis

Fig. 5

A Protein levels and quantification of the levels of FABP3, P-CaMKII, GAPDH and the adipogenic markers C/EBP-α and Perilipin 1 after overexpressing MGP and/or adding KN93, a P-CaMKII inhibitor. B ORO staining and quantitative analysis after infection with OE-NC or OE-MGP lentiviruses and/or the addition of KN93, a P-CaMKII inhibitor. C The protein levels and quantification of GAPDH, P-CaMKII, CaMKII and RIP140 levels are shown along with the concentration gradient of KN93. D CUT&Tag-qPCR was performed with an anti-RIP140 antibody or normal mouse IgG in MSCs infected with OE-NC or OE-MGP lentiviruses. E, F The interaction between MGP or RIP140 and FABP3 was detected through a dual-luciferase reporter system. Scale bar = 50 μm. All the data are presented as the means ± SDs; n = 6 per group. Student’s t test or ANOVA was used to determine significant differences. ns, not statistically significant; *P < 0.05; **P < 0.01; and ***P < 0.001.

Back to article page