Fig. 3: NCOA5 inhibits ferroptosis in HCC cells.
From: NCOA5 induces sorafenib resistance in hepatocellular carcinoma by inhibiting ferroptosis
A Gene Set Enrichment Analysis (GSEA) of ferroptosis-related genes, showing their enrichment in NCOA5 high versus NCOA5 low groups using the GSE182593 dataset. B–D Relative levels of reactive oxygen species (ROS) (B), glutathione (GSH) (C), and malondialdehyde (MDA) (D) in Huh7SR (left panel) and BEL-7402SR (right panel) cells transfected with shNCOA5 #1 compared to shCtrl. **P < 0.01, ***P < 0.001 by Student’s t-test. E Proliferation of Huh7SR (left panel) and BEL-7402SR (right panel) cells, with and without exposure to erastin. F Proliferation of Huh7SR (left panel) and BEL-7402SR (right panel) cells, with and without exposure to RSL3. G Proliferation of both Huh7SR and BEL-7402SR cells transfected with shCtrl compared to those transfected with shNCOA5 #1, with and without exposure to Ferrostatin 1.
