Fig. 6: C12ORF49 enhances lipogenesis by activating SREBP1/SCD1 signaling. | Cell Death Discovery

Fig. 6: C12ORF49 enhances lipogenesis by activating SREBP1/SCD1 signaling.

From: C12ORF49 inhibits ferroptosis in hepatocellular carcinoma cells via reprogramming SREBP1/SCD1-mediated lipid metabolism

Fig. 6

Concentrations of free fatty acid (FFA) (A), triglyceride (TG) (B) and phospholipids (PL) (C) were quantified. D Lipophilic BODIPY 493/503 staining assay was conducted to detect the levels of neutral lipids (Scale bar = 20 µm). The influence of C12ORF49 on the expression levels of SREBP1 and SREBP2 was evaluated by qRT-PCR (E) and WB (F) assays. G WB assay for the impact of C12ORF49 on the nuclear SREBP1 level. H WB assay was conducted for the impact of C12ORF49 on the expression of SCD. FFA (I), TG (J) and PL (K) levels were detected in HCC cells with indicated treatment. L Lipophilic BODIPY 493/503 staining assay was conducted to evaluate the neutral lipids content (Scale bar = 20 µm). M The contents of palmitoleic (C16:1) and oleic (C18:1) acids were detected in HCC cells with indicated treatment. N IHC staining assays for correlations between the expressions of C12ORF49 and SREBP1 and SCD1 in HCC tissues. Values are expressed as mean ± SEM from three individual experiments (n = 3).

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