Fig. 1: Gene expression analysis of a validation cohort of BC patients highlighted a gene expression signature associated with BCG therapy response. | Cell Death Discovery

Fig. 1: Gene expression analysis of a validation cohort of BC patients highlighted a gene expression signature associated with BCG therapy response.

From: Heightened IDO1 levels predict Bacillus Calmette-Guèrin failure in high-risk non-muscle-invasive bladder cancer patients

Fig. 1

A Workflow chart indicating the process to select naïve BC patients according to the response to BCG treatment, retrieved from GSE154261 database (discovery cohort). B Heatmap of differential expressed genes (DEGs) performed with the R edgeR library in responder versus non-responder BC patients (p-value < 0.05). C Enrichment analysis using the EnrichR library in Ontology terms Biological Process, Molecular Function and Cellular Component of DEGs from responder versus non-responder BC patient cohort. D GSEA plot performed, between Non-Responder (NR) vs Responder (R), with the MSigDB library in the C2 class for inflammatory response, TNFA signaling via NFKB, Interferon alpha response and Interferon Gamma Response. E Funnel graph to filter 18,267 DE Genes, starting from 59,000 genes, 1246 genes have a p value < 0.05, and 11 are coding genes with an abs(fc) ≥ 2 (left). Barplot showing 11 top DEGs (right). F Venn diagram showing the intersection between 11 top DEGs and genes belonging to the GO immune signature (n = 1903).

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