Fig. 2: GC-specific AARS2 overexpression induces mouse POI.
From: AARS2-catalyzed lactylation induces follicle development and premature ovarian insufficiency
A–B AARS2 regulates estrous cyclicity. Representative estrous cyclicity of C57, AARS2 GOE, Aars2 GKO, and AARS2 ZOE mice during 15 consecutive days since postnatal 8 weeks (A) and the cycle phase times (n = 16 for AARS2 GOE and 14 for Aars2 GKO and AARS2 ZOE) represented as mean ± SEM (B). C AARS2 affects the litter size. The cumulative number of pups in C57, AARS2 GOE, Aars2 GKO, and AARS2 ZOE was analyzed (n = 6). D–E Levels of FSH (D) and E2 (E) in adult AARS2 GOE and WT mice (16–20 weeks old, n = 14) were measured. F AARS2 does not affect oocyte number. The number of oocytes in C57, AARS2 GOE, and Aars2 GKO mice (n = 6, one ovary from each mouse) was measured at PD1. See also Fig. S2. G AARS2 affects ovarian follicle number. The ovarian follicles in AARS2 GOE, Aars2 GKO, and C57 mice were quantified (n = 6) at PD5, 8, and PD3-, 5-, and 7-weeks. Numbers of primordial (Pri), primary (Pr), type 4 (T4), type 5 (T5), type 6 (T6), and type 7 (T7) follicles were counted (mean ± SEM). See also Fig. S2.
