Fig. 5: Kv1.3-overexpression induced M1 macrophages, Aggravated damage of renal tubular epithelial cells. | Cell Death Discovery

Fig. 5: Kv1.3-overexpression induced M1 macrophages, Aggravated damage of renal tubular epithelial cells.

From: Therapeutic potential of voltage-dependent potassium channel subtype 1.3 blockade in alleviating macrophage-related renal inflammation and fibrogenesis

Fig. 5

A Experimental process flow chart. B Western blotting analysis of Kv1.3, CD86, and CD206 levels after transfection with LV-Kv1.3 in THP-1 cells. C Respective FCM analysis of CD86 and CD206 expression in Kv1.3-overexpressing THP-1 cells. D CBA analysis of the cytokines (IL-6, IL-8, IL-10, IL-1β, TNF-α, IL-12p70) alterations in supernatant of THP-1 cells. E Representative migration images and statistical analysis of Transwell assay. Data were expressed as mean ± SD (n = 4). F Respective western blotting image and densitometry analysis of α-SMA, TGF-β1 and E-cad levels, and normalized to β-Tubulin. Data were expressed as mean ± SD (n = 3).

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