Fig. 10: Overexpressing Elf1 in vivo accelerates NPC senescence to promote IVDD. | Cell Death Discovery

Fig. 10: Overexpressing Elf1 in vivo accelerates NPC senescence to promote IVDD.

From: ELF1-mediated transactivation of METTL3/YTHDF2 promotes nucleus pulposus cell senescence via m6A-dependent destabilization of E2F3 mRNA in intervertebral disc degeneration

Fig. 10

A Immunofluorescence detection of Elf1 protein expression patterns in NP tissues after injection of different doses of Elf1 AAV5. B, C Western blot and qPCR analyses were used to detect the protein and mRNA expression levels of Elf1, Mettl3, and Ythdf2 in NP tissues after Elf1 overexpression. D, G, H MRI detection of the T2-weighted signal intensity and Pfirrmann grading of intervertebral discs in mice after injection of Elf1 AAV5; N = 6. E, F, I H&E and Safranin-O staining and histological scoring of mouse intervertebral discs after injection of Elf1 AAV5; N = 6; Scale bars = 100 μm and 20 μm. J, K Immunofluorescence detection of the protein expression patterns of Elf1, p21, and Mettl3 and the m6A abundance in the NP tissues of mice treated with an injection of Elf1 AAV5. L Western blot analysis was used to detect the protein expression levels of Collagen II, Adamts5, Mmp13, Cyclin D1, and p21 in M_NPC after Elf1 overexpression. M Western blot analysis was used to detect the protein expression levels of Mettl3 and E2f3 after MMF treatment in Elf1-overexpressing M_NPC. N Molecular mechanisms of ELF1-mediated regulation of NPC senescence. The data are presented as the mean ± SD. One-way ANOVA was used for comparisons among multiple groups. *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001.

Back to article page