Fig. 2: Reducing the expression of ELF1 inhibits the senescence of NPC and delays the IVDD. | Cell Death Discovery

Fig. 2: Reducing the expression of ELF1 inhibits the senescence of NPC and delays the IVDD.

From: ELF1-mediated transactivation of METTL3/YTHDF2 promotes nucleus pulposus cell senescence via m6A-dependent destabilization of E2F3 mRNA in intervertebral disc degeneration

Fig. 2

A Heatmap of differentially expressed genes in ELF1+ cell subpopulations vs. ELF1- cell subpopulations. B The knockdown efficiency of ELF1 siRNA was detected by qPCR. C Western blot detection of protein expression levels of ADAMTS5, MMP13 and P21 in H_NPC after knockdown of ELF1. D The mRNA expression levels of extracellular matrix genes and cell cycle genes were detected by qPCR after H_NPC knockdown of ELF1. E, F Flow cytometry was used to detect the cell cycle progression of H_NPC after knockdown of ELF1. G, H EDU assays the DNA replication capacity of H_NPC after knockdown of ELF1. I, J Immunofluorescence was used to detect the protein expression level of P21 after the addition of ELF1 siRNA after 10 ng/ml IL-1β treatment of H_NPC for 48 h; N = 3 biologically independent replicates; Scale bar = 50 μm. K, L Representative images of SA-β-gal staining and quantification after 10 ng/ml IL-1β treatment of H_NPC for 48 h followed by the addition of ELF1 siRNA; N = 3 biologically independent replicates. Scale bar = 100 μm. M Western blot for protein expression levels of p21, Mmp13 and Collagen II in NP tissue after Elf1 KO. N The mRNA expression levels of extracellular matrix genes and cell cycle genes were detected by qPCR in NP tissues after Elf1 KO. O, R MRI detection of T2-weighted signal intensity in the intervertebral discs of WT and Elf1 KO naturally aging mice of different months of age; N = 6; P, Q, S H&E and Safranin-O staining of intervertebral discs from WT and Elf1 KO naturally aging mice at different months of age; N = 6; Scale bar = 100 μm and 20 μm. T, U Representative x-ray film images of intervertebral disc heights in WT and Elf1 KO naturally aging mice at different months of age; N = 6. V Immunofluorescence detection of the expression level of p16 in the NP tissues of intervertebral discs of WT and Elf1 KO naturally aging mice at different months of age. Data presented as mean ± SD. One-way ANOVA was used for comparison among multiple groups. *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001.

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