Fig. 3: METTL3 is involved in the regulation of NPC senescence as a downstream of ELF1.

A Distribution of Peak and TSS distances. Vertical coordinates are samples, different colors represent Peak in different intervals from TSS, horizontal coordinates are the proportion of Peak in different intervals to the total Peak, Upstream means Peak is upstream of TSS, Downstream means Peak is downstream of TSS. B Peak binding spectrum of TSS region. When the Peak coverage frequency of the TSS region is in the shape of a symmetrically distributed peak, it indicates that the target protein is concentrated in the TSS region. C Peak Neighbouring gene KEGG taxonomic annotations. D Venn diagram shows the intersection of m6A regulator with RNA-seq differential gene and Chip_seq_nearest_genes gene. E Volcano plot demonstrating the expression level of METTL3 in RNA-seq. F, G Immunofluorescence detection of m6A modification and Mettl3 expression levels in medullary tissues of naturally aging mice. H Immunofluorescence detection of Mettl3 expression levels in NP tissues in a rat model of acupuncture IVDD. I Immunohistochemical detection of METTL3 expression in human NP tissue. J The expression levels of METTL3 and P21 were detected 48 h after 10 ng/ml IL-1β treatment. K, L Immunofluorescence detection of METTL3 expression levels in the NPC replicative senescence model. M The knockdown efficiency of METTL3 was detected by qPCR. N The knockdown efficiency of METTL3 was detected by Western Blot. O, P Flow cytometry to detect the effect of knockdown of METTL3 on NPC cell cycle progression. Q, R EDU assays the effect of knockdown of METTL3 on NPC DNA replication. S Effect of knockdown of METTL3 on mRNA expression of cell cycle genes detected by qPCR. T, U SA-β-gal staining was used to detect the effect of knockdown of METTL3 on NPC senescence after 48 h of 10 ng/ml IL-1β treatment. Data presented as mean ± SD. One-way ANOVA was used for comparison among multiple groups. *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001.