Fig. 2: Inhibition of GSDMD by LDC7559 suppressed breast cancer to lung metastasis in 4T1 bearing mice. | Cell Death Discovery

Fig. 2: Inhibition of GSDMD by LDC7559 suppressed breast cancer to lung metastasis in 4T1 bearing mice.

From: Mitochondrial reactive oxygen species promote cancer metastasis and tumor microenvironment immunosuppression through gasdermin D

Fig. 2

a Scheme of drug treatment strategy. 4T1 cells were injected into fourth mammalian pat of Balb/c mice for two weeks, and LDC7559 was performed daily for 2 weeks. b Quantitative analysis of tumor growth curve with and end-point tumor volume at day 28 in 4T1 and LDC7559 treated tumor model. Arrow in 14 days is the first time of LDC7559 treatment. c Photograph of excised solid tumor taken on day 28. d Photograph of spleen from 4T1 and LDC7559 treated 4T1 bearing mouse model extracted at day 28. e Quantitative analysis of the weight of spleen in control, 4T1 and LDC7559 treated 4T1 bearing mouse group. f Photographs of lungs from control, 4T1 and LDC7559 treated 4T1 bearing mouse model extracted at day 28. Scale bar, 1 cm. g Representative hematoxylin-eosin staining (H&E) staining of 4T1 metastasis tumor area in the lung tissue sections. Scale bar, 1 mm. h The 4T1 metastasis tumor area was calculated in (g). j qPCR analysis of IL-1β, IL-18 and HMGB-1 mRNA level in lung (GAPDH was used as an endogenous reference for qPCR). k ELISA analysis of IL-1β, IL-18 and HMGB-1 protein levels in lung from 4T1 and LDC7559 treated tumor model. l ELISA analysis of MPO-DNA and NE-DNA level in serum samples from 4T1 and LDC7559 treated tumor model. n = 6 mice. Data are presented as means ± SEM. Significance was examined with one-way ANOVA (e, h, i), or two-tailed unpaired Student’s t test (b, j, k, l). ns not significant, *P < 0.05, **P < 0.01, ****P < 0.0001.

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