Fig. 3: Inhibition of GSDMD by LDC7559 relieved immunosuppression in 4T1 bearing mice.

a Flow cytometry analysis scheme of G-MDSC (CD45⁺CD11b⁺Ly6G⁺), M-MDSC (CD45⁺CD11b⁺Ly6C⁺), CD4⁺ T cells (CD45⁺CD4⁺) and CD8⁺ T cells (CD45⁺CD8⁺) in lung of indicated groups. b Quantification of the percentages of G-MDSC, M-MDSC, CD4⁺ T cells and CD8⁺ T cells in (a). c Quantification of absolute number of G-MDSC, M-MDSC, CD4⁺ T cells and CD8⁺ T cells in per lobe of lung in indicated groups. d Representative flow cytometric plots of peripheral blood G-MDSC and M-MDSC in wild type mice, 4T1-bearing mice, or LDC7559 treated 4T1-bearing mice. e Quantification of the percentages of G-MDSC and M-MDSC in (d). f Quantification of absolute number of G-MDSC and M-MDSC in (d). g Representative immunofluorescence analysis of Gr1 and CD8 in lung from wild type mice, 4T1-bearing mice, or LDC7559 treated 4T1-bearing mice. Scale bar, 100 μm. Cell numbers of Gr1⁺ cells (h) and CD8⁺ T cells (i) were calculated in (g). n = 5 mice. Data are presented as means ± SEM. Significance was examined with two-tailed unpaired Student’s t test (b, c, e, f) or Student’s t test (h, i). ns not significant, ^*P < 0.05, ^**P < 0.01, ^***P < 0.001.