Fig. 2: MiRNA signature effect on different types of cell death after TMZ treatment. | Cell Death Discovery

Fig. 2: MiRNA signature effect on different types of cell death after TMZ treatment.

From: Androgen receptor inhibition sensitizes glioblastoma stem cells to temozolomide by the miR-1/miR-26a-1/miR-487b signature mediated WT1 and FOXA1 silencing

Fig. 2

A Analysis of Annexin V/PI by flow cytometry in the indicated GBM neurospheres overexpressing the miRNA signature mimics or negative control mimic (neg. ctrl) after 6 days of TMZ treatment with the indicated doses. Upper panel: representative experiment of the percentage of apoptotic cells. Lower panel: percentage of Annexin positive cells in TMZ-treated neurospheres overexpressing the miRNA signature compared to controls; B Western blots of a representative experiment of MLKL, p-MLKL expression (necroptosis-related markers) and GPX4 (ferroptosis-related marker) in the indicated GBM neurospheres overexpressing the miRNA signature with respect to control and treated with TMZ. C Analyses of cell viability by Cyto3D™Live-Dead assay and western blots of a representative experiment of MLKL and p-MLKL expression in the indicated GBM neurospheres overexpressing the miRNA signature and treated concomitantly with TMZ and NSA. Densitometric analyses by ImageJ software are shown. *p < 0.05; **p < 0.01.

Back to article page