Fig. 3: Identification and validation of WT1 and FOXA1 as miR-1-3p, miR-26a-1-3p, and miR-487b-3p direct targets. | Cell Death Discovery

Fig. 3: Identification and validation of WT1 and FOXA1 as miR-1-3p, miR-26a-1-3p, and miR-487b-3p direct targets.

From: Androgen receptor inhibition sensitizes glioblastoma stem cells to temozolomide by the miR-1/miR-26a-1/miR-487b signature mediated WT1 and FOXA1 silencing

Fig. 3

A Ingenuity pathway analysis by Qiagen tool of genes differentially expressed in TMZ-resistant compared to—sensitive GBM cells (GSE131781); B Left panel: Schematic representation of the predicted miRNA target genes interaction with androgen receptor (AR) by STRING analysis; right panel: scatter plots of the Pearson’s correlation of gene expression between WT1 and FOXA1 with AR in 1018 GBM patients from the CGGA RNAseq dataset. Correlation coefficient (R) and linear trend line are reported; C Upper panel: Schematic representation of putative binding sites of miR-1, miR-26a-1 in the 3′UTR of WT1 gene, and of miR-487b in the 3’UTR of FOXA1 gene. Lower panel: Firefly luciferase activity in recipient cells after transient co-transfection with Renilla luciferase reporter plasmid containing the 3′UTR of the indicated gene target sites, and the relative miRNA-mimic or ctrl-mimic. Results are expressed as fold activation relative to the basal activity of psiCHECK2 empty control; D WT1- and FOXA1-mRNA and related protein expression levels in the indicated GBM neurospheres after miRNAs overexpression. Densitometric analyses by ImageJ software are shown. All the values are reported as the mean of at least three experiments. Error bars indicate the standard deviation. *p ≤ 0.05, **p ≤ 0.01.

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