Fig. 2: Knockdown of CCNE1 inhibits TNBC cell growth and metastasis in vitro and in vivo. | Cell Death Discovery

Fig. 2: Knockdown of CCNE1 inhibits TNBC cell growth and metastasis in vitro and in vivo.

From: CCNE1 stabilizes ANLN by counteracting FZR1-mediated the ubiquitination modification to promotes triple negative breast cancer cell stemness and progression

Fig. 2

The proliferative ability of BT-549 and MDA-MB-231 cells transfected with lentivirus shCtrl or shCCNE1 was evaluated by (A) MTT assay and (B) cell clonal formation assay, respectively. C Cell apoptosis of BT-549 and MDA-MB-231 following CCNE1 knockdown was measured by flow cytometry. D The migration of BT-549 and MDA-MB-231 cells transfected with lentivirus shCtrl or shCCNE1 was evaluated by wound-healing assay. E The invasion of BT-549 and MDA-MB-231 cells transfected with lentivirus shCtrl or shCCNE1 was evaluated by Transwell assay. n = 3, **P < 0.01, ***P < 0.001. MDA-MB-231 cells into the right forelimb axillary of nude mice. F On day 26, the mice were placed in the In Vivo Imaging System for total fluorescence intensity of mice tumor. G After tumor formation, body weight of mice was recorded, and tumor dimensions were measured twice weekly using digital calipers during the experimental period, respectively. H After the mice tumor tissue protein was extracted and performed WB analysis to detected the expression of CCNE1 in shCCNE1 groups and shCtrl groups. I Typical images of HE staining and immunohistochemistry staining of mice tumor tissue in shCCNE1 groups and shCtrl groups. n = 5, *P < 0.05, ***P < 0.001.

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