Fig. 2: SOX4 promotes proliferation, colony formation, and angiogenesis in vitro and tumor growth in vivo. | Cell Death Discovery

Fig. 2: SOX4 promotes proliferation, colony formation, and angiogenesis in vitro and tumor growth in vivo.

From: SOX4 reprograms fatty acid metabolism through the CHREBP to inhibit ferroptosis in hepatocellular carcinoma

Fig. 2

A, B CCK-8 assays show that SOX4 knockdown (HepG2 cells) reduces cell proliferation, while overexpression (Huh7 cells) enhances proliferation. C, D EDU assays confirm decreased proliferation rates upon SOX4 knockdown and increased rates with SOX4 overexpression. E, F Colony formation assays show reduced colony formation in SOX4 knockdown cells and enhanced formation in overexpressing cells. G, H Fluorescence-labeled angiogenesis assays reveal SOX4’s role in promoting angiogenesis in vitro. I, J Xenograft experiments show reduced tumor growth and volume in SOX4 knockdown cells and increased tumor growth in overexpressing cells. K Immunohistochemistry further validates SOX4 protein levels in tumor tissues.*P < 0.05, **P < 0.01, ***P < 0.001.

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