Fig. 2: Overexpression of miR-139-5p promotes ferroptosis in glioma cells. | Cell Death Discovery

Fig. 2: Overexpression of miR-139-5p promotes ferroptosis in glioma cells.

From: miR-139-5p activates ferroptosis by inhibiting the expression of HMG-CoA reductase to inhibit the progression of glioma

Fig. 2

A, E, I, M, Q qRT-PCR showed that 50 nM miR-139-5p mimic was transfected into U251, U138 and PGC#1 cells and increased the expression of miR-139-5p. On the contrary, miR-139-5p inhibitor decreased the expression of miR-139-5p in LN229 cells. Results are normalized to U6 and GAPDH. B, F, J, N The MDA content in U251, U138, PGC#1 and LN229 cells was detected using an MDA kit. C, G, K, O The GSH content in U251, U138, PGC#1 and LN229 cells was detected using a GSH kit. D, H, L, P The Flow cytometry histogram showed the lipid peroxidation level of U251, U138, PGC#1 and LN229 cells. Q CCK-8 assay revealed the proliferative activity of U251, U138, PGC#1 and LN229 cells under different treatments. Data are presented as mean ± standard deviation (SD) from three independent experiments. ***p < 0.001, **p < 0.01, *p < 0.05.

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