Fig. 4: miR-139-5p promotes ferroptosis by inhibiting the HMGCR expression. | Cell Death Discovery

Fig. 4: miR-139-5p promotes ferroptosis by inhibiting the HMGCR expression.

From: miR-139-5p activates ferroptosis by inhibiting the expression of HMG-CoA reductase to inhibit the progression of glioma

Fig. 4

A The binding fragments of miR-139-5p and HMGCR gene were predicted by the TargetScanHuman database. B–E qRT-PCR assay revealed that the expression of miR-139-5p in U251 and U138 cells transfected with miR-139-5p overexpressed lentivirus was upregulated and the expression of HMGCR was downregulated compared to that in the control group. F U251 and U138 cells were treated with 100 μM MVA for 48 h, and the expression levels of related proteins were detected by western blotting. Results of western blotting of U251 and U138 cells were statistically analysed. G, H, K, L The relevant kit was used to detect MDA, GSH & GSSG levels in the U251 and U138 cells. I, M Flow cytometry was used to detect the lipid peroxidation in U251 and U138 cells. Statistical results of lipid peroxidation level in U251 and U138 cells were analysed. J, N Proliferative activity of U251 and U138 cells was analysed using the CCK-8 assay. Data are presented as mean ± standard deviation (SD) from three independent experiments. ***p < 0.001, **p < 0.01, *p < 0.05.

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