Fig. 5: miR-139-5p synergistically promotes ferroptosis and inhibits cholesterol synthesis with simvastatin. | Cell Death Discovery

Fig. 5: miR-139-5p synergistically promotes ferroptosis and inhibits cholesterol synthesis with simvastatin.

From: miR-139-5p activates ferroptosis by inhibiting the expression of HMG-CoA reductase to inhibit the progression of glioma

Fig. 5

A U251 and U138 cells were treated with 10 μM SIM for 48 h, and the expression levels of related proteins were detected by western blotting. Results of western blotting of U251 and U138 cells were statistically analysed. B, C, F, G The relevant kit was used to detect MDA, GSH levels in the U251 and U138 cells. D, H Flow cytometry was used to detect the lipid peroxidation levels in U251 and U138 cells. Statistical results of lipid peroxidation detection in U251 and U138 cells. E, I Proliferative activity of U251 and U138 cells was analysed using the CCK-8 assay. J The relevant kit was used to detect cholesterol levels in the U251 and U138 cells. K LC–MS/MS was employed to quantitatively analyze the intracellular levels of mevalonic acid (MVA). Data are presented as mean ± standard deviation (SD) from three independent experiments. ***p < 0.001, **p < 0.01, *p < 0.05.

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