Fig. 1: TREM2 deficiency impaired cognitive function in PD mice.

A Representative image of TREM2 (red) and IBA1 (green) immunofluorescence staining in hippocampal tissue sections of the PD and control specimens. Scale bar, 50μm. B Representative image of TREM2 (red) and IBA1 (green) immunofluorescence staining in hippocampus in C57BL/6 mice and A53T mice. Scale bar, 50 μm. C Quantification of number of TREM2⁺ IBA1⁺ cells in PD specimens and control specimens (n = 3, unpaired Student’s t test) and TREM2⁺ IBA1⁺ cells in C57BL/6 mice and A53T mice (n = 3, unpaired Student’s t test). D Recognition Index was calculated in NORT (n = 9, one-way ANOVA and Šídák’s multiple comparisons test). E Representative image showing the procedure and swimming paths obtained from each group in the acquisition phase in MWM. F Time spent in the target quadrant (s) in the acquisition phase in MWM (n = 9, one-way ANOVA and Šídák’s multiple comparisons test). G Representative image showing the paths obtained from each group in the acquisition phase in Open field test. Line chart showing the average escape latencies in each group in the space exploring phase in MWM (n = 9, two-way ANOVA and Tukey’s multiple comparisons test). Column charts showing. The error bars represent the ± SDs. **p < 0.01, ***p < 0.001. NORT, novel object recognition test; MWM, Morris water maze.