Fig. 2: TREM2 deficiency exacerbated α-Syn accumulation and lysosomal dysfunction in the hippocampus.

A Representative images of western blotting analysis of Oligomer α-Syn, p-α-Syn, and α-Syn expressions in each group. B Quantification of Oligomer α-Syn, P-α-Syn, and α-Syn proteins relative expression (n = 3, one-way ANOVA and Šídák’s multiple comparisons test). C Representative images of α-Syn (red) and IBA1 (green) in the hippocampus of each group. Scale bar, 400μm. D Quantification of mean Fluorescence intensity of α-Syn in the hippocampus of each group (n = 3, unpaired Student’s t test). E Quantification of IBA1 within 20 μm of α-Syn (%) in the hippocampus of each group (n = 3, unpaired Student’s t test). F Representative images of IBA1 (red) and CD68 (green) immunofluorescence staining in the hippocampus of each group. Scale bar, 50μm. G Quantification of IBA1 colocalized with CD68 (%) in the hippocampus of each group (n = 3, one-way ANOVA and Šídák’s multiple comparisons test). H Representative images of western blotting analysis of CathB, CathD, and Lamp1 expressions in each group. I Quantification of CathB, CathD, and Lamp1 proteins relative expression (n = 3, one-way ANOVA and Šídák’s multiple comparisons test). J Representative images of CathB (red) and IBA1 (green) immunofluorescence staining in the hippocampus of each group. Scale bar, 10μm. K Representative images of Lamp1 (purple) and IBA1 (green) immunofluorescence staining in the hippocampus of each group. Scale bar, 10μm. L Quantification of area CathB colocalized with IBA1 (%) in the hippocampus of each group (n = 3, one-way ANOVA and Šídák’s multiple comparisons test). M Quantification of IBA1 colocalized with Lamp1 (%) in the hippocampus of each group (n = 3, one-way ANOVA and Šídák’s multiple comparisons test). The error bars represent the ± SDs. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.