Fig. 6: Knockdown of TREM2 aggravated α-Syn phosphorylation and apoptosis in HT22 cells. | Cell Death Discovery

Fig. 6: Knockdown of TREM2 aggravated α-Syn phosphorylation and apoptosis in HT22 cells.

From: TREM2 deficiency exacerbates cognitive impairment by aggravating α-Synuclein-induced lysosomal dysfunction in Parkinson’s disease

Fig. 6

A Coculture of BV-2 cells and HT22 cells. B Representative images of flow cytometry of HT22 cells in each group. C Representative images of TEM of apoptotic morphology of HT22 cells in each group. D Quantification of apoptosis of HT22 cells in each group (n = 3, one-way ANOVA and Šídák’s multiple comparisons test). E Representative images of p-α-Syn (red) immunofluorescence staining in HT22 cells in each group. Scale bar, 10μm. F Quantification of mean Fluorescence intensity of p-α-Syn in HT22 cells in each group (n = 3, one-way ANOVA and Šídák’s multiple comparisons test). The error bars represent the ± SDs. ***p < 0.001, ****p < 0.0001. PFFs, recombinant human A53T α-Syn pre-formed fibrils; TEM, transmission electron microscopy.

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