Fig. 3: LMNB2 is a bona fide transcription factor of PD-L1.
From: LMNB2-mediated high PD-L1 transcription triggers the immune escape of hepatocellular carcinoma
A RNA-seq analysis of control and LMNB2 knockdown Huh7 cells. B Volcano map of DEGs in control and LMNB2 knockdown Huh7 cells. C GSEA analysis of PD-L1 signaling in control and LMNB2 knockdown Huh7 cells. D Correlation analysis between LMNB2 and PD-L1 expression in the TIMER database. E Correlation analysis between LMNB2 and PD-L1 expression in the GEPIA database. F Correlation analysis between LMNB2 and immune cell infiltration in HCC using the TIMER database. G LMNB2 promotes the protein expression of exogenous PD-L1 in HepG2 cells. H LMNB2 promotes the protein expression of endogenous PD-L1 in HepG2 cells. I Model diagram of LMNB2 binding to the PD-L1 promoter to promote its transcription. J LMNB2 promotes the mRNA expression of PD-L1. K LMNB2 promotes the activity of PD-L1. L Model diagram of the six truncated PD-L1 promoters. M WB verified the overexpression of LMNB2 in 293T cells for dual-luciferase reporter analysis. N Statistical of the LMNB2 effect on the activity of six truncated PD-L1 promoters (O). Distribution of LMNB2 in ST-RNA-seq of HCC samples. P Correlation analysis between LMNB2 and PD-L1 in ST-RNA-seq in HCC samples. Q IHC staining of LMNB2 and PD-L1 in HCC samples. Scale bar, 200 μm. R Correlation analysis between LMNB2 and SPOP IHC scores. Data are shown as mean ± SD (n ≥ 3). ***p < 0.001, ****p < 0.0001.
