Fig. 4: The inhibitor combination can cause apoptosis in cells via DNA damage. | Cell Death Discovery

Fig. 4: The inhibitor combination can cause apoptosis in cells via DNA damage.

From: Combined inhibition by PRMT5 and MAT2A demonstrates a strong synthetic lethality in MTAP homozygous-deficient glioma models

Fig. 4

A Brightfield of LN18, U87 and U251 cell lines after treatment with the 2 inhibitors (PRMT5i: 10 nM; MAT2Ai: 10 nM) (scale bar: 100 μm). B Comet assay in LN18, U87 and U251 cell lines after treatment with the 2 inhibitors (PRMT5i: 10 nM; MAT2Ai: 10 nM) (scale bar: 100 μm). C, D Flow cytometry to determine the number of apoptotic cells in LN18, U87 and U251 cells after inhibitor treatment (PRMT5i: 10 nM; MAT2Ai: 10 nM), and their statistical charts (n = 3). One-way ANOVA for multi-group comparisons. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. E Western blotting to determine changes in protein expression trends of BAX and BCL-2 in LN18, U87 and U251 cells (PRMT5i: 10 nM; MAT2Ai: 10 nM), and their statistical charts (n = 3). F, G Immunofluorescence assay to determine the effects of the 2 inhibitors (PRMT5i: 10 nM; MAT2Ai: 10 nM) in the LN18, U87 and U251 cell lines using TUNEL staining (scale bar: 100 μm), and their statistical charts (n = 3). One-way ANOVA for multi-group comparisons. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.

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