Fig. 5: The combination of PRMT5 and MAT2A inhibitors produces synthetic lethal effects in glioma organoid models.

A Immunofluorescence assay to determine MTAP expression in 2 glioblastoma organoids, and their statistical charts (scale bar: 200 μm) (n = 3). B Flowchart of organoid experiments. C, D Brightfield images showing the cellular state of organoids after treatment with the inhibitors at 7 days and 14 days (scale bar: 200 μm). E, F H&E staining and immunohistochemical staining of SDMA, Ki67, γ-H2AX using paraffin sections of glioblastoma organoids after 14 days of co-culture (scale bar: 200 μm).