Fig. 5: KLF15 hypermethylation in fibrotic kidneys and activated fibroblasts. | Cell Death Discovery

Fig. 5: KLF15 hypermethylation in fibrotic kidneys and activated fibroblasts.

From: Inhibition of epigenetic regulator UHRF1 attenuates renal fibrosis and retains transcription factor Krüppel-like factor 15 expression

Fig. 5

A, B MeDIP-qPCR analysis shows the level of renal KLF15 promotor methylation in UUO mice or in UIR mice. The left panel shows a virtual gel of PCR products of methylated DNA and input DNA. The right panel shows quantitative real-time PCR results. n = 4 mice per group. C Relative renal KLF15 mRNA expression in UUO mice or in UIR mice, analyzed by qRT-PCR. n = 5 mice per group. D, E Relative renal KLF15 protein expression in UUO mice or in UIR mice, analyzed by Western blots. n = 6 mice per group. F–J NRK-49F cells were treated with 10 ng/ml TGF-β1 for 48 h. And KLF15 promotor methylation was analyzed by MeDIP-qPCR (F, G). Relative KLF15 mRNA expression, analyzed by qRT-PCR (H). Relative KLF15 protein expression, analyzed by Western blots (I, J). K, L NRK-49F cells were infected with CRISPR-Cas9 lentivirus targeting KLF15. Relative KLF15, α-SMA and Fibronectin protein expression were analyzed by Western blots. n = 3 samples per group. Data were shown as mean ± SEM. Data were analyzed using unpaired Student’s t test. *P < 0.05, **P < 0.01.

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