Fig. 6: Knockdown of UHRF1 inhibits fibroblasts activation by demethylating KLF15 and restoring its expression. | Cell Death Discovery

Fig. 6: Knockdown of UHRF1 inhibits fibroblasts activation by demethylating KLF15 and restoring its expression.

From: Inhibition of epigenetic regulator UHRF1 attenuates renal fibrosis and retains transcription factor Krüppel-like factor 15 expression

Fig. 6

A MeDIP-qPCR analysis shows the level of KLF15 promotor methylation. NRK-49F cells were transfected with UHRF1 siRNA or control siRNA before treatment of 10 ng/ml TGF-β1 for 48 h. n = 3 samples per group. B, C Col1a2-Cre+/UHRF1flox/flox mice (fibroblast-specific depletion of UHRF1) and their littermate controls received Sham or UUO operation for 7 days. B Renal KLF15 promotor methylation levels were determined by MeDIP-qPCR. n = 4 mice per group. C Renal KLF15 protein levels were determined by Western blots. n = 6 mice per group. Data were shown as mean ± SEM. Data were analyzed using unpaired Student’s t test (B) or One-way ANOVA followed by Tukey (A, C). *P < 0.05, **P < 0.01.

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