Fig. 1: NETosis is involved in viral pneumonia with excessive inflammatory cytokines.
From: Hippo pathway and NLRP3-driven NETosis in macrophages: Mechanisms of viral pneumoniaaggravation
A, B Comparative analysis of NETosis biomarkers in neutrophils from peripheral blood of patients with viral pneumonia versus healthy volunteers via immunofluorescence. C Graphic presentations of fluorescence mean densities of NETosis biomarkers. D Assessment of CitH3 protein expression in whole blood sample from patients with viral pneumonia and healthy volunteers using western blotting. E Quantitative analysis of the protein CitH3 relative to Tubulin. F Quantification of serum dsDNA and LL-37 levels conducted through Enzyme linked immunosorbent assay (ELISA). G Evaluation of serum pro-inflammatory cytokines, including TNF-α, IL-1β, and GM-CSF, via ELISA. All data are representative as means ± s.e.m of three independent experiments. Student’s t test for A-G; *p < 0.05; **p < 0.01; ***p < 0.001. Scale bar = 50 μm.
