Fig. 3: Activation of the Hippo pathway in macrophages following poly(I:C) stimulation in mice.

A A simplified schematic diagram illustrating the interactions between components of the Hippo pathway. B Expression of Hippo pathway expression in lung tissues from mice treated with poly(I:C) compared to untreated controls. C The protein expression of Hippo pathway were quantified using densitometry on ImageJ. Phospho-LATS1 and LATS1, phospho-MST and MST1, phospho-MST and MST2, phospho-YAP and YAP protein were normalized to Tubulin and the ratio is presented. D Relative expression of YAP protein in lung tissues from mice treated with or without poly(I:C) was assessed after nuclear-plasma separation. E Quantitative analysis of the protein YAP in nuclear relative to Lamin B1. F The levels of inflammatory cytokines and chemokines in BALF. G The levels of inflammatory cytokines and chemokines in serum. H YAP and neutrophils colocalization in peripheral blood of mice infected with poly(I:C). Scale bar = 50 μm. I, J Colocalization analysis of YAP with macrophages in peripheral blood (H) and BALF (I) from poly(I:C) stimulated mice. Scale bar = 20 μm. K Quantification of the colocalization between Ly6G or F4/80 and YAP. L Expression of the Hippo pathway in macrophages from mice treated with or without poly(I:C). M Quantification of phospho-LATS1 and LATS1, phospho-MST and MST1, phospho-MST and MST2, phospho-YAP and YAP protein in macrophages from mice were normalized to Tubulin and the ratio is presented. N Relative expression of YAP protein in macrophages following nuclear-plasma separation from mice treated with or without poly(I:C). O Quantitative analysis of the protein YAP in nuclear relative to Lamin B1. All data are representative as means ± s.e.m of three independent experiments. Student’s t test for (A–I); *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001.