Fig. 4: Activation of the Hippo Pathway in Macrophages Enhances NETosis under poly(I:C) stimulation.
From: Hippo pathway and NLRP3-driven NETosis in macrophages: Mechanisms of viral pneumoniaaggravation
A Relative expression of nuclear YAP protein in RAW246.7 cells treated with or without poly(I:C) was assessed, following nuclear-plasma separation. B Quantitative analysis of the protein YAP in nuclear relative to Lamin B1. C Hippo pathway expression in RAW246.7 cells treated with or without poly(I:C). D Quantification of phospho-LATS1 and LATS1, phospho-MST and MST1, phospho-MST and MST2, phospho-YAP and YAP protein in macrophages from mice were normalized to Tubulin and the ratio is presented. E–H Neutrophils were cultured with conditioned medium collected from RAW 264.7 cells that had been treated under different conditions. Neutrophils were collected and level of CitH3 and MPO were assessed using immunofluorescence (E) CitH3 protein expression was assessed using Western blotting (F, G). dsDNA and LL-37 levels were evaluated by ELISA (H). Note: IN, Lats-IN-1 is a potent and ATP-competitive inhibitor of LATS1 and LATS2 kinases. All data are representative as means ± s.e.m of three independent experiments. Student’s t test for (A–H); *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001. Scale bar = 20 μm.
