Fig. 8: Hippo pathway regulates macrophage IL-1β secretion via NLRP3.
From: Hippo pathway and NLRP3-driven NETosis in macrophages: Mechanisms of viral pneumoniaaggravation
A Box plot of the expression levels of Cxcl5, Nlrp3, and Yap1 between the virus-induced lung injury and control group. B Box graph of NETosis related genes using ssGSEA enrichment scores between the virus-induced lung injury and control group. C Correlation heat map. D–F The efficiency of knockdown and overexpression of NLRP3 via lentivirus vectors were assessedusing PCR and Western blotting detection. G Levels of NLRP3 mRNA in macrophages isolated from the BALF of mice and in RAW264.7 cells with or without poly(I:C) stimulated. H, I L-1β expression in macrophages with or without NLRP3 regulation was evaluated using Western blotting. J Quantitative analysis of the protein IL-1β relative to Tubulin under different conditions. All data are representative as means ± s.e.m of three independent experiments. Student’s t test for (A–I); *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001. Scale bar = 20 μm.
