Fig. 5: ER stress is triggered upon NCA stimulation. | Cell Death Discovery

Fig. 5: ER stress is triggered upon NCA stimulation.

From: Neocarzilin A induces apoptosis and mitochondrial disturbance by targeting reticulon 4-mediated endoplasmic reticulum stress

Fig. 5

A Subcellular fractionation of HeLa cells treated with NCA or DMSO as indicated with subsequent immunoblotting for ER chaperone BiP. B ER stress markers (eIF2α + ATF4) of HeLa cells treated with NCA or DMSO as indicated, Thapsigargin (TG) served as positive control. C CHOP mRNA levels of HeLa cells treated with NCA or DMSO as indicated for 18 h analyzed by qPCR, TG served as positive control. Relative quantification via ΔΔCt method. A–C Data are presented in bar graphs as mean ± SD, n = 3. Statistical significance was analyzed by A two-tailed unpaired Student’s t-test or B, C one-way ANOVA with Dunnett’s posttest compared to mean of B wt group or C DMSO control (ns not significant, *P < 0.05, **P < 0.01, ***P < 0.001).

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