Fig. 6: Induction of intrinsic apoptosis pathways by NCA. | Cell Death Discovery

Fig. 6: Induction of intrinsic apoptosis pathways by NCA.

From: Neocarzilin A induces apoptosis and mitochondrial disturbance by targeting reticulon 4-mediated endoplasmic reticulum stress

Fig. 6

A–F Analysis of apoptosis markers by immunoblotting. HeLa cells were treated with NCA or DMSO as indicated and ratio of cleaved to total A caspase 8, B Bid and F PARP or relative expression of C caspase 9, E caspase 3 were determined. D Cytochrome c release was analyzed by subcellular fractionation and immunoblotting in mitochondrial and cytosolic fractions. G DNA fragmentation was analyzed by propidium iodide staining and flow cytometry. A-G Data are presented in bar graphs as mean ± SD, n = 3. Statistical significance was analyzed by A, B, C, E, F, G one-way ANOVA with Dunnett’s posttest or D Mann-Whitney test (ns=not significant, *P < 0.05, **P < 0.01, ***P < 0.001).

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