Fig. 2: Network construction of lncRNA-microRNA-mRNA. | Cell Death Discovery

Fig. 2: Network construction of lncRNA-microRNA-mRNA.

From: DNMBP-AS1/hsa-miR-30a-5p/PGC1α axis suppresses tumor progression of colorectal cancer by inhibiting PKM2-mediated Warburg effect and enhance anti-PD-1 therapy efficacy

Fig. 2

A Volcano map of differentially expressed lncRNAs of COAD and READ in TCGA |log2(fold change)| ≥ 0.5 or ≤ 0.5; adj. p value < 0.05). Blue dots: significantly downregulated (Down); red dots: significantly upregulated (Up); gray dots: no significant differences (NS). B lncRNAs of miRNAs (miR-30a-5p and miR-23a-3p) were identified using miRNet and starBase. C The miRNA-lncRNA interaction pairs were visualized using Cytoscape. D, E Co-expression correlation analysis of miRNA-lncRNA and mRNA-lncRNA. F Overall survival of five lncRNA in TCGA-COAD/READ cohort. G, H The wild-type and the mutated sequences of PGC1α and DNMBP-AS1 mRNA 3’-UTR (mutation site: red). I, J The luciferase activity of CRC cells (SW620 and HT29) in luciferase reporter plasmid containing wild-type PGC1α 3’-UTR (PGC1α-WT) and mutant PGC1α 3’-UTR (PGC1α-MUT) or wild-type KCNQ1OT1 3’-UTR (KCNQ1OT1-WT) and mutant KCNQ1OT1 3’-UTR (KCNQ1OT1- MUT) co-transfected with miR-30a-5p mimics or negative control was assessed, **p < 0.01. K Diagram of the DNMBP-AS1/hsa-miR-30a-5p/PGC1α ceRNA network.

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