Correction to: Cell Death Discovery https://doi.org/10.1038/s41420-024-02213-x, published online 17 October 2024
First, our description of the sentence “The results showed that immunoprecipitation of RIPK2 could pull down the N4BP3 and Ub-K63 proteins, but not the Ub-K48 protein” is inaccurate. And it should be revised to “The result showed that immunoprecipitation of RIPK2 could pull down N4BP3 and K63-linkage specific ubiquitin-linked N4BP3, but not K48-linkage specific ubiquitin-linked N4BP3”.
For the molecular weights of Ub-K63 and Ub-K48 in Figure 6C and RIPK2 in Figure 6A, our markings do need to be changed, and we have uploaded both the revised figure 6 and original full length western blots of figure 6 in the attachment.
Second, we apologize that we mischaracterized some words and misquoted a reference in the discussion section.
“XIAP activates the NOD2 pathway by promoting the ubiquitination of RIPK2-M1 connection” should be modified to “XIAP activates the NOD2 pathway by promoting the ubiquitination of RIPK2-K63 connection”.
RNF31 does activate the NF-κB pathway through M1-linked ubiquitination, so RNF31 should be excluded from “and TRAF4, RNF31, TRIM27, and RNF34 inhibit the NOD2 pathway by promoting the ubiquitination of RIPK2-K48 connection [38,39,40,41]”.
Original full length western blots of figure 6.
Figure 6. N4BP3 interacts with RIPK2 and promotes its ubiquitination. [A] After inducing HCT116 cells with MDP (25 μg/mL) for 4h, immunoprecipitation of the anti-N4BP3 antibody can pull down N4BP3, NEDD4, and RIPK2, but not P-P65. [B] After co-transfecting GFP-N4BP3 plasmid, Flag-RIPK2 plasmid, and His-Ub plasmid and inducing HCT116 cells with MDP (25 μg/mL) for 4h, immunoprecipitation of the anti-Flag antibody can pull down the His antibody for a larger range. [C] After inducing HCT116 cells with MDP (25 μg/mL) for 4h, immunoprecipitation of the anti-RIPK2 antibody can pull down RIPK2, N4BP3, and K63-linkage specific ubiquitin-linked N4BP3, but not K48-linkage specific ubiquitin-linked N4BP3.
The original article has been corrected.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.
About this article
Cite this article
Jiang, W., Zhao, Y., Han, M. et al. Correction: N4BP3 facilitates NOD2-MAPK/NF-κB pathway in inflammatory bowel disease through mediating K63-linked RIPK2 ubiquitination. Cell Death Discov. 11, 408 (2025). https://doi.org/10.1038/s41420-025-02574-x
Published:
DOI: https://doi.org/10.1038/s41420-025-02574-x
