Fig. 3: MEF2A bound to PD-L1 promoter to transcriptionally upregulate PD-L1 expression. | Cell Death Discovery

Fig. 3: MEF2A bound to PD-L1 promoter to transcriptionally upregulate PD-L1 expression.

From: N6-methyladenosine modification of MEF2A weakens cetuximab sensitivity in colorectal cancer via PD-L1/SOX12 axis

Fig. 3

The binding motif for MEF2A (A) and MEF2A binding sequences at PD-L1 promoter region (B) were forecasted through JASPAR database. Luciferase reporter assay (C), CHIP (D), and EMSA (E) for analyzing the binding of MEF2A protein on PD-L1 promoter in CRC cells. F, G sh-MEF2A or MEF2A overexpression vector was transfected into CRC cells. RT-qPCR (bF) and western blot (G) for testing PD-L1 mRNA and protein expressions. All bar chart analysis of western blot is a repeated experiment three times. *p < 0.05, **p < 0.01, and ***p < 0.001.

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