Fig. 5: PD-L1 bound to SOX12 mRNA and stabilized its expression. | Cell Death Discovery

Fig. 5: PD-L1 bound to SOX12 mRNA and stabilized its expression.

From: N6-methyladenosine modification of MEF2A weakens cetuximab sensitivity in colorectal cancer via PD-L1/SOX12 axis

Fig. 5

A PD-L1 protein pulled down by biotinylated SOX12 probe in RNA pull-down assay. B The co-localization of SOX12 mRNA and PD-L1 was measured using FISH combined with immunofluorescence. Scale bar: 25 μm. Representative immunofluorescence (C) and FISH (D) images in the negative control experiments. Scale bar: 25 μm. E RIP for detecting the binding relationship between PD-L1 and SOX12 mRNA. F Actinomycin D was added into CRC cells transfected with sh-PD-L1 or PD-L1 overexpression vector. SOX12 mRNA expressions were tested using RT-qPCR. G SOX12 level in CRC was analyzed using bioinformatics analysis (UALCAN). H SOX12 mRNA levels in normal tissues (n = 20), cetuximab-sensitive (n = 8) and cetuximab-resistant (n = 12) CRC tissues were detected with RT-qPCR. I Western blot for determining SOX12 protein level in CRC cells. All bar chart analysis of western blot is a repeated experiment three times. *p < 0.05, **p < 0.01, and ***p < 0.001.

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