Fig. 4: The endometrium of Dcaf13 cKO female mice exhibited abnormal responses to estrogen and progesterone.
From: DCAF13 is essential for mouse uterine function and fertility
A The expression level of estrogen receptor ESR1 in the uterus of control and Dcaf13 cKO mice. B Immunohistochemical quantification shows a significant reduction in the expression of ESR1 in the endometrial luminal epithelial cells and stromal cells of Dcaf13 cKO mice. C qPCR analysis revealed a significant downregulation of Esr1 in Dcaf13 cKO mice. D–G qPCR results show a significant downregulation of mRNA levels of estrogen-responsive genes in the uterus of Dcaf13 cKO mice. H The expression level of PGR in the uterus. I Immunohistochemical quantification shows a significant upregulation of PGR expression in endometrial luminal epithelial cells of Dcaf13 cKO mice, but a significant downregulation in endometrial stromal cells. The enlarged images in c and d correspond to the red boxes in images a and b, with scale measurements of 200 μm for a and b, and 50 μm for c and d. LE luminal epithelium, S stroma. *P < 0.05; **P < 0.01; ***P < 0.001.
