Fig. 6: DCAF13 regulates H3K9me3 level by interacting with SUV39H2.
From: DCAF13 is essential for mouse uterine function and fertility
A qPCR results show that the mRNA levels of SUV39H2 in siDCAF13#1 and siDCAF13#4 groups are significantly higher compared to control cells. B Western Blotting was used to detect the expression level of SUV39H2. C After transfecting siDCAF13#1 and siDCAF13#4 into Hela cells, the expression of the SUV39H2 protein was significantly upregulated. D, E. The interaction between DCAF13 protein and SUV39H2 protein was demonstrated through IP and Western Blotting. F Analysis of the H3K9me3 level via Western Blotting after siDCAF13#1 and siDCAF13#4 transfection. G The quantification of H3K9me3 detected by Western Blotting. ***P < 0.001. H A proposed model suggests that DCAF13 plays an essential role in maintaining the morphology, structure, and function of the uterus. In normal circumstances, DCAF13 facilitates the polyubiquitination of SUV39H2, which helps in maintaining low levels of H3K9me3 and promotes the transcription of necessary genes for normal uterine function. However, the knockdown of DCAF13 leads to the accumulation of SUV39H2, resulting in elevated H3K9me3 levels, and inhibits transcription, ultimately leading to abnormal uterine morphology, structure, and function.
