Fig. 2: shRNA-mediated FOXA1 knockdown promotes proliferation, migration, and invasion in NPC cells. | Cell Death Discovery

Fig. 2: shRNA-mediated FOXA1 knockdown promotes proliferation, migration, and invasion in NPC cells.

From: Targeting the FOXA1/BMI1 axis to overcome chemoresistance and suppress tumor progression in nasopharyngeal carcinoma

Fig. 2

A Efficient knockdown of FOXA1 in CNE1 and CNE2 cells was achieved via lentiviral shRNA delivery, and the corresponding reduction in protein levels was confirmed by Western blot analysis. B The CCK-8 assay was employed to assess the impact of FOXA1 knockdown on the growth of CNE1 and CNE2 cells. C A colony formation assay was conducted to evaluate the proliferative potential of FOXA1-silenced CNE1 and CNE2 cells. D Wound healing assays were utilized to examine the migratory capacity of CNE1 and CNE2 cells following FOXA1 knockdown. E, F The migratory and invasive capabilities of FOXA1-depleted CNE1 and CNE2 cells were determined using Transwell migration and Matrigel invasion assays, respectively. Data are presented as the mean ± SD from three replicates. **P < 0.01, #P < 0.001, as determined by one-way ANOVA test.

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