Fig. 4: FOXA1 negatively regulates BMI1 expression in NPC. | Cell Death Discovery

Fig. 4: FOXA1 negatively regulates BMI1 expression in NPC.

From: Targeting the FOXA1/BMI1 axis to overcome chemoresistance and suppress tumor progression in nasopharyngeal carcinoma

Fig. 4

A Immunohistochemical (IHC) experiments revealed a significant negative correlation between FOXA1 and BMI1 protein expression in NPC patient samples. Representative images illustrate varying levels of BMI1 expression in NPC tissues corresponding to high or low FOXA1 expression. Scale bar = 50 μm. B Following FOXA1 knockdown in CNE1 and CNE2 cells, the protein expression levels of BMI1 and the histone modification marker H2AK119ub1 were assessed using western blot analysis. C The impact of FOXA1 knockdown on BMI1 promoter activity was evaluated using a dual-luciferase reporter assay. D Chromatin immunoprecipitation polymerase chain reaction (ChIP-PCR) assays confirmed the binding of FOXA1 to a specific region within the BMI1 gene promoter. E Western blot analysis revealed an inverse correlation between FOXA1 and BMI1 expression in NPC cells, where FOXA1 knockdown led to increased BMI1 levels and a corresponding elevation of the BMI1-dependent histone modification H2AK119ub1. To elucidate the role of BMI1 in FOXA1-mediated functions, BMI1 knockdown was induced in FOXA1-silenced NPC cells, resulting in a significant reduction of H2AK119ub1, as confirmed by western blotting. Data are represented as the mean ± SD. *P < 0.05 and #P < 0.001.

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