Fig. 2: AR abrogation, MUC1-C de novo expression and STAT3 activation in 22Rv1-RR cells. | Cell Death Discovery

Fig. 2: AR abrogation, MUC1-C de novo expression and STAT3 activation in 22Rv1-RR cells.

From: Decoding MUC1 and AR axis in a radiation-induced neuroendocrine prostate cancer cell-subpopulation unveils novel therapeutic targets

Fig. 2

A Total protein levels of AR full length-FL (110 kDa), MUC1 (130 kDa) and MUC1-C (25 kDa) in 22Rv1-P and -RR cells submitted to 5, 10, 15 and 20 fractions of 2.5 Gy. β-actin (42 kDa) was used as loading control. Relative mRNA expression levels of (B) AR-FL and (C) MUC1 in 22Rv1-P and -RR cells. Results are presented as mean ± SD of at least three independent experiments. GUSB was used as reference gene for normalization. D Protein-protein interaction of MUC1 regulatory network. Data was retrieved from TRRUST v2 online platform (https://www.grnpedia.org/trrust/). E Relative mRNA expression levels of STAT3 in 22Rv1-P and -RR cells. Results are presented as mean ± SD of at least three independent experiments. GUSB was used as reference gene for normalization. F Total protein levels of γ-STAT3 (86 kDa) in 22Rv1-P and -RR subpopulations. β-actin (42 kDa) was used as loading control. Western blot images (A, F) were acquired by Chemidoc detection system (Bio-Rad, USA).

Back to article page