Fig. 3: Huaier induces downregulation of the SLC7A11/GPX4 axis. | Cell Death Discovery

Fig. 3: Huaier induces downregulation of the SLC7A11/GPX4 axis.

From: Huaier suppresses lung cancer by simultaneously and independently inhibiting the antioxidant pathway SLC7A11/GPX4 while enhancing ferritinophagy

Fig. 3

a Heatmap illustrating the differentially expressed proteins involved in the ferroptosis pathway in Huaier-treated vs control A549 cells. Samples are labeled as C 1–3 (Control 1–3), H 1-3 (Huaier 1–3). b Western blot analysis of GPX4 expression in A549, H1975, and H358 cells following treatment with Huaier at concentrations of 0, 5, 7.5, and 10 mg/ml for 48 h. c Western blot analysis of SLC7A11 expression in A549, H1975, and H358 cells treated with Huaier at 5 mg/ml for 48 h. A549, H1975, and H358 cells were stably transfected with SLC7A11 plasmid and subsequently treated with Huaier at 5 mg/ml for 48 h. Cystine uptake (d) and Lipid peroxidation (e) were assessed. Scale bar: 5 μm. Data are shown as mean ± SD, n = 3, *p < 0.05, **p < 0.01, ***p < 0.001. f A549, H1975, and H358 cells, stably transfected with SLC7A11 plasmid, were treated with varying Huaier concentrations of Huaier (0, 1, 3, 5, 7.5, 10 mg/ml) for 48 h, and the inhibition ratio was determined using a CCK-8 assay. g In A549 and H358 cells, stably transfected with the SLC7A11 plasmid and treated with Huaier at 5 mg/ml for 48 h, Western blot analysis was performed to evaluate SLC7A11 and GPX4 protein levels.

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