Fig. 5: 4EBP1 contributes to the tumorigenic potential of MYC-amplified MB cell lines. | Cell Death Discovery

Fig. 5: 4EBP1 contributes to the tumorigenic potential of MYC-amplified MB cell lines.

From: The EIF4EBP1 gene encoding 4EBP1 is transcriptionally upregulated by MYC and linked to shorter survival in medulloblastoma

Fig. 5

A, B Control (ishCtrl) or stable inducible 4EBP1 knockdown (ish4EBP1#1 and #2) Med8A (A) or HD-MB03 (B) cells were treated with 1 μg/ml doxycycline and grown in soft agar for 21 days. Colonies and single cells were counted, and colony formation efficiency was calculated and normalized to control. Data are reported as means ± SD of three individual replicates with indicated significance. Protein expression of MYC and 4EBP1 was analyzed by immunoblotting. Significance was calculated using an unpaired and two-tailed parametric t test (***p < 0.001, ****p < 0.0001). C, D Control (ishCtrl) or stable inducible 4EBP1 knock down (ish4EBP1#1 and #2) Med8A (C) or HD-MB03 (D) were treated with 1 μg/ml doxycycline and cell proliferation was measured with an EdU assay. Data are reported as means ± SD of three individual replicates. Statistics were calculated using an unpaired and two-tailed parametric t test (n.s. = not significant).

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