Fig. 1: Nerve and cancer cells interact with each other in a timedependent manner in the in vitro PNI model. | Cell Death Discovery

Fig. 1: Nerve and cancer cells interact with each other in a timedependent manner in the in vitro PNI model.

From: Defining the cellular and molecular features of nerve-invaded cancer cells using a newly characterized experimental model

Fig. 1

A–C Bright field images show time dependent tropism of cancer cells (red arrows: LASCPC-01, LNCaP, MDA-MB-231) toward nerve endings on days 5 and 12 in the in vitro PNI model (scale bar, 500 µm). A closer view of the nerve-cancer cell interface on day 12 is also shown on the far right (scale bar, 100 µm). (D) Nerve-cancer cell interface shows emerging SCs (white arrow) from the nerve endings migrating towards cancer cells (red arrow) (scale bar, 500 µm). E- top A closer view of the boxed region in ‘D’ shows the presence of SCs immediately outside the nerve endings in the in vitro PNI model (scale bar, 100 µm). E-bottom A combination of brightfield and fluorescent images of the field presented in ‘E- top’ shows the presence of GFP+ cancer cells (yellow arrows) migrating towards the nerve endings along the SC bed (scale bar, 100 µm).

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