Fig. 3: JIB-04 triggers proliferation and migration defects by modulating ERK-mediated signaling, thus phenocopying MECOM silencing. | Cell Death Discovery

Fig. 3: JIB-04 triggers proliferation and migration defects by modulating ERK-mediated signaling, thus phenocopying MECOM silencing.

From: Epigenetic targeting of MECOM/KRAS axis by JIB-04 impairs tumorigenesis and cisplatin resistance in MECOM-amplified ovarian cancer

Fig. 3

A, B MECOM-amplified SKOV3 and OVSAHO cells were treated with E-JIB-04 (0.7 µM; IC50) and Z-JIB-04 (6 µM; IC50) and were allowed to form colonies for 10 days. Significant reduction in survival fraction of treated cells was observed. C Western blot analysis resulted in reduction in phosphorylation of ERK1/2 upon treatment with E- and Z- forms of JIB-04 in SKOV3 cells. Bar graph provides the quantification of densitometric analysis of protein bands. D Realtime qPCR analysis revealed downregulation of EGR1, downstream signaling molecule of ERK in JIB-04 treated SKOV3 cells. E Inhibitory effect of E- and Z-forms of JIB-04 on SKOV3 cell migration using the transwell migration assay. Bar graph provides the quantification of percentage cell migration. Quantitative RT-qPCR of pro-migratory markers Vimentin, Fibronectin, N-cadherin, and EMT marker ZEB1 upon treatment of SKOV3 cells with E-JIB-04 (F) and Z-JIB-04 (G). Uncropped western blot images corresponding to Fig. 3C were shown in Supplementary information.

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