Fig. 6: Impact of stress stimuli on T cells, B cells and NK cells within the TME.

a UMAP plot of T cells, B cells and NK cells in the TME, grouped by cell subtypes. b Proportion of each subtype of T, B, and NK cells. Statistical significance was indicated (Fisher’s exact test, **p < 0.01). c Heatmap of the average expression levels of the marker genes used for annotation of each subtype of T, B, and NK cells. d Proportion of each subtype of T, B, and NK cells in all cells. e GO pathway enrichment analysis of DEGs in T cells showing the top 10 pathways (adjusted p < 0.05). f GSEA plot showing representative pathways including leukocyte proliferation, T cell differentiation, T cell activation involved in immune response and T cell-mediated cytotoxicity in T cells (MS vs NC). g The signature enrichment score (U score) of representative pathways including leukocyte proliferation, T cell differentiation, T cell activation involved in immune response and T cell mediated cytotoxicity in T cells (MS vs NC). h CD4 and CD8 immunofluorescence staining analysis of tumor tissue sections. Quantification of the CD4 staining (i) and CD8 staining (j) in (h) (mean ± SEM, n = 5). k GO pathway enrichment analysis of DEGs in B cells showing the top 10 pathways (adjusted p < 0.05). l GSEA plot showing representative pathways including B cell proliferation, B cell activation, and B cell-mediated immunity in B cells (MS vs NC). m Bubble plot displaying the top 10 significantly downregulated pathways (MS vs NC) in GSEA analysis of NK cells. n GSEA plot showing representative pathways including NK cell-mediated immunity, NK cell activation, and NK cell-mediated cytotoxicity in NK cells (MS vs NC).