Fig. 5: Obatoclax enhanced ER stress and apoptosis via ATF-4-mediated regulation in GBM. | Cell Death Discovery

Fig. 5: Obatoclax enhanced ER stress and apoptosis via ATF-4-mediated regulation in GBM.

From: Inhibition of anti-apoptotic Bcl-2 family members promotes synergistic cell death with ER stress inducers by disrupting autophagy in glioblastoma

Fig. 5

A PERK pathway-related expression in Onda7 and DK-MG cell lines was detected via Western blotting 24 h after treatment with 1 µM obatoclax, 1 µM tunicamycin, or both. B The expression of the ATF-4-Bim-CHOP axis in Onda7 and DK-MG cell lines was detected via Western blotting after indicated treatment with 2.5 nM siATF-4 and combination therapy with 1 µM obatoclax and 1 µM tunicamycin. C Caspase 3/7 activity of Onda7 and DK-MG was measured using the Caspase-Glo 3/7 assay after combining treatment with 100 nM obatoclax and 200 nM tunicamycin or treatment with 2.5 nM siATF-4. Results are presented as mean fold change ± SD (n = 6). ****P < 0.0001. D Additional ER stress pathway-related expression in Onda7 and DK-MG cell lines were detected via Western blotting 24 h after treatment with 1 µM obatoclax, 1 µM tunicamycin, or both.

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