Fig. 2: The engagement of WTAP in DDR.

A The efficiency of WTAP knockdown and protein level of γH2AX in two HCC cell lines detected by western blot. B Representative images and quantification of tail DNA observed in siCON or siWTAP cells by comet assay (n = 50, Mann-Whitney test). Scale bar, 500 μm. C Immunofluorescence analysis of the formation of γH2AX foci in siCON or siWTAP HCC cells (n = 50, Mann-Whitney test). Scale bar, 20 μm. D HR and NHEJ efficiency of siCON or siWTAP HCC cells detected by flow cytometry. All results were obtained from at least three independent experiments. **p < 0.01, ***p < 0.001 compared with siCON.