Fig. 2: Analysis of cellular communication and macrophage phenotype in the testicular microenvironment of iNOA patients.

A UMAP clustering diagram of macrophage distribution in testicular tissue of iNOA patients and control group. B Analysis of CD74 gene expression in testicular macrophages. C Based on the UMAP map of Seurat reclustering, a total of 11 cell groups were subdivided. D Based on the inflammation gene set score in MsigDB, the horizontal axis represents cell clustering, and the vertical axis represents the inflammation score value. E The distribution of macrophage subtypes (pro-Mφ and anti-Mφ) in the testicular tissue of the control group and iNOA group. F The proportion of pro-Mφ and anti-Mφ in the whole macrophage in the testis of the control group and iNOA group. The analysis of intercellular communication in the control group, including communication between pro-Mφ and different spermatogenic cells (G), mutual communication between cell groups (H), and communication relationship between pro-Mφ and ligand-receptor of spermatogenic cells (I). The analysis of intercellular communication in the iNOA group includes the communication between pro-Mφ and different spermatogenic cells (J), the mutual communication between cell groups (K), and the communication relationship between pro-Mφ and ligand-receptor of spermatogenic cells (L). Signal patterns output (M) by SPG, pro-Mφ, and SSC cells and signal patterns received (N) by them in the iNOA group.